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The discovery in the mid-1950's of gas-liquid chromatography (GLC) has revolutionized the analysis of fatty acids and, undoubtedly, this technique is the most frequently used. Indeed, for the quantification of individual fatty acids in any acylated lipids, GLC must be adopted.
A (FAME) can be created by an alkali catalyzed reaction between fats or fatty acids and methanol. Since every microorganism has its specific FAME profile, it can be used as a tool for microbial source tracking (MST).
The types and proportions of fatty acids present in cytoplasm membrane and outer membrane (gram negative) lipids of cells are major phenotypic traits. The technique for determining these fatty acids is called FAME (Fatty acid methyl ester).

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