DNA sequencing is the most accurate and reliable method for microbial identification for most organisms. This application is ideal for situations where a rapid and accurate identification is mandatory; results can be obtained rapidly(please call for details). We can identify organisms whether they are viable, non-viable, stressed or healthy. Phenotypic systems are limited to viable organisms. Royal's clients benefit because we use FAME analysis, DNA sequencing and microscopic analysis for identifications. These tools together complement each other to create a well rounded and accurate identification system.
DNA-based microbial identification for bacteria, actinomycetes, fungi and yeasts
Fully validated library entries for bacteria, actinomycetes, yeasts, and fungi
Sample comparisons by means of a phylogenetic tree Biochemical/Fame/16 S rDNA
Sample comparison to GenBank, an international DNA sequence database
Identify yeast, mold, or bacteria through DNA sequencing for an isolate
Options for same day, next day, two day and three day turn around times
Custom Phylogenetic Trees
A Phylogenetic Tree is generated based on the differences between the sequences of library entries and/or client samples. Base pair differences between at least five organisms are weighted differently to determine a percent similarity. The distances in the branches of the tree are mathematically calculated based on these percents and adjusted accordingly.
This tool can be very helpful when trying to determine the level of mutation imposed on a specific sample when compared to the original strain. This can also be a valuable asset in trending the changes within a species or genus over time. Records obtained from the gene sequencing procedure are always available to assist in the ease of accessing past data .
